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| Registros recuperados: 67 | |
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| Bon, Fabienne; Ambert Balay, K; Giraudon, H; Kaplon, J; Le Guyader, Soizick; Pommepuy, Monique; Gallay, A; Vaillant, V; De Valk, H; Chikhi Brachet, R; Flahaut, A; Pothier, P; Kohli, E. |
| We compiled sequence and epidemiological data from 172 caliciviruses detected in France from December 1998 to February 2004 in sporadic and outbreak cases. The results showed a cocirculation of strains with a majority of genogroup II (GII) noroviruses. Three groups of noroviruses, not detected before in our laboratory, emerged and spread during the period: the recombinant GGIIb and Norwalk-related strains not amplified in the polymerase gene in 2000 and a new Lordsdale variant in 2002. We observed that (I) GII4 noroviruses were predominant in nursing home and hospital outbreaks but rare in oyster- and water-related outbreaks despite continuous circulation in the population; (ii) at the opposite, genogroup I strains were detected in the majority of... |
| Tipo: Text |
Palavras-chave: Genogroup; Cocirculation; Gastroenteritis; Caliciviruses; Epidemiological. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-723.pdf |
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| Le Guyader, Soizick; Pommepuy, Monique; Atmar, Robert. |
| Human and animal fecal wastes and urine contain a large number of different viruses that can enter the environment through the discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. Viruses are present in shellfish in very low numbers. Nevertheless, they are present in sufficient quantities to pose a health risk as presented. This low level of contamination has made it necessary to develop highly sensitive viral extraction methods to ensure virus recovery from shellfish tissues. The most common route for accidental contamination is after heavy... |
| Tipo: Text |
Palavras-chave: Human enteric viruses; Shellfish; Sewage; Persistence; Flux. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12606/15279.pdf |
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| Pommepuy, Monique; Le Saux, Jean-claude; Hervio Heath, Dominique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish like oysters, mussels, cockles and clams, have long been identified. Bacterial diseases such as cholera and typhoid fever were the first to be suspected of being linked to consumption of contaminated shellfish (Butt et al., 2004). During the past century, various strategies have been established in shellfish growing areas throughout the world to assure the sanitary quality of shellfish. More recently, despite sanitary surveys, Vibrio parahaemolyticus, Vibrio vulnificus and enteric viruses - especially Hepatitis A virus (HAV) and norovirus (NoV) – were found to be associated in outbreaks of human illness. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12607/9485.pdf |
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| Coutard, Francois; Pommepuy, Monique; Loaec, Solen; Hervio Heath, Dominique. |
| Aims: This work investigates the maintenance of viability and potential virulence of Vibrio parahaemolyticus in a viable but nonculturable population (VBNC) state by reverse transcription-polymerase chain reaction (RT-PCR). Methods and Results: Housekeeping genes, 16S-23S rDNA and rpoS, as well as virulence genes, tdh1 and tdh2, were selected and detected by PCR in a pathogenic strain of V. parahaemolyticus (Vp4). Their expression was then studied by RT-PCR in V. parahaemolyticus Vp4 cultivated in rich medium at 37degreesC. The 16S-23S rDNA and rpoS, tdh1, tdh2 genes were transcripted at the mid-logarithmic, stationary and late stationary phases, corresponding to various physiological states. The expression of these genes was also studied by RT-PCR in a... |
| Tipo: Text |
Palavras-chave: Vibrio parahaemolyticus; Viable but nonculturable state; Tdh2; Tdh1; RT PCR; RpoS; Environment; 16S 23S rDNA. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1098.pdf |
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| Le Guyader, Soizick; Loisy, Fabienne; Atmar, R; Hutson, A; Estes, M; Ruvoen-clouet, N; Pommepuy, Monique; Le Pendu, J. |
| The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha... |
| Tipo: Text |
Palavras-chave: Bioaccumulation; Shellfish; Escherichia coli; Pathogen; Oyster digestive tissue; Noroviruses. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1542.pdf |
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| Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique. |
| Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between... |
| Tipo: Text |
Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf |
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| Caprais, Marie-paule; Le Mennec, Cecile; Pommepuy, Monique; Le Saux, Jean-claude; Gourmelon, Michele. |
| In Europe, shellfish harvesting areas are classified according European Directive EC N°854/2004, using classic faecal indicator levels (Escherichia coli). A better indicator is needed, however, because shellfish may contain other human pathogens. Other indicator organisms have therefore been evaluated and, among these, coliphages meet most of the requirements to serve as indicators and models for enteroviruses. In order to evaluate the capacity of coliphages as indicators, a total of 764 shellfish were sampled in 2004 from 70 shellfish harvesting areas monitored by the Ifremer microbiological surveillance network (REMI, http://www.ifremer.fr/envlit/), and tested for F-specific RNA bacteriophages (FRNAPH). Two species of bivalve mollusc were collected... |
| Tipo: Text |
Palavras-chave: F-specific RNA bacteriophage; Shellfish; Faecal pollution; Discrimination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17749/15271.pdf |
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| Pommepuy, Monique. |
| Les zones marines littorales reçoivent des rejets d'eaux usées d'origine urbaine et agricole qui contiennent de très grandes quantités de micro-organismes dont certains sont pathogènes pour l'homme ; dans environnements hostiles, la dispersion et les conditions vie défavorables font que ces bactéries et virus disparaissent rapidement ou évoluent vers un état non cultivable. Cependant, certains milieux - sédiments, eaux turbides ou eutrophisées présentent favorables à la survie bactéries et à la protection des particules virales, Les travaux réalisés ces dernières ont apporté la preuve que bactéries sont capables de survivre dans l'environnement, d'adapter leur métabolisme aux conditions naturelles et sur très longues périodes ; dans ces conditions évoluent... |
| Tipo: Text |
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| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00040/15158/12494.pdf |
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| Pommepuy, Monique. |
| INTRODUCTION Problématique générale Les zones marines côtières reçoivent des rejets d'eaux urbaines et agricoles qui contiennent de grandes quantités de micro-organismes dont certains sont pathogènes pour l'homme (par ex. virus de l'hépatite A. ..). Dans les environnements hostiles, la dispersion et conditions de survie défavorables (énergies lumineuse importante, eau oligotrophe...) font que les bactéries disparaissent rapidement ou évoluent vers un état non cultivable. Par contre, certains milieux comme les sédiments, les eaux turbides ou eutrophisées, présentent des conditions favorables à la survie des bactéries d'origine entérique et à la protection physique des particules virales. Dans les écosystèmes perturbés de nombreux apports anthropiques, la... |
| Tipo: Text |
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| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00040/15117/12453.pdf |
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| Coutard, Francois; Lozach, Solen; Pommepuy, Monique; Hervio Heath, Dominique. |
| A real-time reverse transcription-PCR method was developed to determine whether the recovery of culturability of viable but nonculturable (VBNC) Vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type III secretion system 2 (TTSS2). The culturability of clinical strain Vp5 of V. parahaemolyticus in artificial seawater at 4°C was monitored, and the VBNC state was obtained. One day after entry into the VBNC state, temperature upshifts to 20 and 37°C allowed the recovery of culturability. Standard curves for the relative quantification of expression of the housekeeping genes rpoS, pvsA, fur, and pvuA; the tdh2 gene; and the TTSS2 genes (VPA1354, VPA1346, and VPA1342) were established. The... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2787.pdf |
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| Gourmelon, Michele; Montet, Mp; Loaec, Solen; Le Mennec, Cecile; Pommepuy, Monique; Vernoy-rozand, C. |
| La qualité sanitaire du milieu marin côtier est influencée par la présence de rejets urbains ou de rejets diffus d'origine agricole. Ces effluents sont riches en bactéries ou virus d'origine fécale, dont certains ont été mis en cause dans des pathologies humaines (Beeching, 1997 ; Feldhusen, 2000 ; Lipp et Rose, 1997 ; Yam et al., 1999). Parmi les microorganismes pouvant présenter un danger pour l'homme, certains, pathogènes émergents ces dernières années, n'ont été que récemment recherchés dans l'environnement : on peut citer les Cryptosporidium et les Giardia pour les parasites, les norovirus pour les virus et les Escherichia coli vérotoxiques (VTEC) pour les bactéries. Ces derniers ne sont connus que depuis 1982, date à laquelle E. coli 0157 : H7 a été... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00106/21697/19276.pdf |
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| Pommepuy, Monique; Dupray, Elisabeth; Guillaud, Jean-francois; Derrien, A; L'Yavanc, Jacky; Cormier, M. |
| The behaviour of fecal bacteria was investigated in a turbid estuary which is also a shellfishing area. Sediments are polluted by sewage by the way of settlement, fecal bacteria mixed with estuarine water being able to survive a very long time (T90 are about several days). By measuring the increase of salt tolerance of the strains grown in natural estuarine organic matter, it was demonstrated that Escherichia coli is able to support the salinity of coastal water. Moreover, because light penetration is obstructed by suspended matter, the solar bactericidal effect is very low. A close relationship between T90 and light intensity received by the bacteria is found. These mechanisms can explain the low die-off rate found in turbid areas. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00267/37860/35941.pdf |
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| Troussellier, Marc; Bonnefont, Jean-luc; Courties, Claude; Derrien, Annick; Dupray, Elizabeth; Gauthier, Michel; Gourmelon, Michele; Joux, Fabien; Lebaron, Philippe; Martin, Yvan; Pommepuy, Monique. |
| The effects of different environmental factors (nutrient deprivation, hyperosmotic shock, exposure to light) on enteric bacteria which have been transferred into the marine environment, have been studied experimentally (microcosms) by considering demographic, physiological and genetic responses in Escherichia coli or Salmonella typhimurium populations. Short-term experiments (less than or equal to 48 h) showed that nutrient deprivation induced limited changes in measured bacteriological variables, but when combined with hyperosmotic shock, it results in an energy charge decrease and inactivation of membrane transport. Light exposure mainly affects the colony-forming capacity of bacterial populations. Combining different stress factors confirmed the rapid... |
| Tipo: Text |
Palavras-chave: Genetics; Cellular states; Physiology; Seawater; Enteric bacteria; Génétique; Etats cellulaires; Physiologie; Eau de mer; Entérobactéries. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-845.pdf |
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| Pommepuy, Monique. |
| Conformément aux termes d'un contrat passé entre le CNEXO et le Service Départemental de l’Équipement, l’Unité Littoral Centre Océanologique de Bretagne a effectué de janvier à septembre 1977, plusieurs campagnes océanographiques en rade de BREST. Les objectifs pouIsuivis etaient 1 - d'estimer la dispersion des eaux fluviales dans la rade pendant des marées de vives-eaux et de mortes-eaux et durant des périodes de crue et d'étiage des rivières 2 - de suivre, sur la tranche d'eau, en plusieurs points de la rade et au cours d'un cycle complet de marée, l'évolution des paramètres suivants: courant, température, salinité, sels nutritifs, matières en suspension; 3 - de tracer les trajectoires probables d'un rejet hypothétique en un point donné et à une heure... |
| Tipo: Text |
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| Ano: 1977 |
URL: http://archimer.ifremer.fr/doc/00055/16578/14080.pdf |
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| Registros recuperados: 67 | |
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